Volume 7. Number 1


Год / Year


Volume 7 Number 1 2024



Environmental Concerns and the State of Aquatic Environment

Hydrometeorological factors and their impact on the chlorophyll-α content in the Azov Sea in 2006–2022    

Kochergin A.T.


Background. Chlorophyll-α concentration is a key environmental parameter used to calculate the primary production of a water body, which, in turn, determines phytoplankton biomass. The fields with increased chlorophyll concentration can develop under the influence   of   various   hydrometeorological   factors considered in this work, such as air and water temperatures, cloud amount, wind-driven water mixing, and precipitation   affecting   the   surface   water   layer.   Relevance.   Monitoring   of   chlorophyll   distribution as it is influenced by hydrometeorological factors facilitates the rational fisheries management on account of their relation to the phytoplankton biomass providing fish and zooplankton   with   a food   source. The aim of this work is to analyze the inter-annual variability of the average monthly hydrometeorological factors and their influence on the chlorophyll-α concentration in   the Azov Sea (excluding Taganrog Bay) for the time range from 2006 to 2022. Methods. Spatial and temporal analysis of the average monthly water temperatures and chlorophyll-α concentrations has been conducted based   on   the   daily   satellite images of the sea surface taken by MODIS spectroradiometer in the Azov Sea; the analysis of the meteorological characteristics has been carried out using the weather   observation data   from international data exchange and reports (SYNOP, METAR) posted on the website pogodaiklimat.ru for the time range from April 2006 to September 2022. Results. It is shown that, for the entire investigated time range (2006–2022), the trends of the inter-annual variability of the average monthly air and water temperatures were not significant. An increase in water temperature that started in the middle of 1990s changed for the opposite in 2011, i. e. turned into a decrease. The wind velocity above the Azov Sea (excluding Taganrog Bay) over the investigated time range gradually decreased, which fell in line with the decrease in the chlorophyll-α concentrations. The average monthly cloud amount in the area has shown   a weak inter- annual and considerable seasonal variability with relatively low values in the summer season and high values in the winter season. A positive trend of the monthly amount of atmospheric precipitation was observed, in contrast with the negative trend of the chlorophyll-α concentration. Conclusion. A significant positive correlation of the average monthly   chlorophyll-α values   with   water   and   air temperatures   has been identified, as well as a negative one with the wind velocity and cloud amount over the Azov Sea (excluding Taganrog Bay)—both in the individual investigated areas and in the sea as a whole.

Keywords:   air   temperature,   sea   surface   temperature,   wind   velocity,   cloud   amount,    precipitations,

chlorophyll-α,   correlation

Biology and Ecology of Aquatic Organisms

The effect of certain pollutants on nectobenthic crustaceans    

Trigub A.G., Medyankina M.V., Drozdenko T.V., Khayrulina T.P.


Background. Ingress of pollutants in water bodies, both direct and from the catchment area, is constantly increasing. It creates an escalating threat to aquatic ecosystems and humans as consumers of water and aquatic biological resources, which contributes to the   relevance   of   the   monitoring   and   regulation   of water pollution. This work is aimed at the evaluation of the effect of various toxic substances and pollutants on the survival of the amphipod   Hyalella   azteca   in   a   chronic   experiment.   Methods. This study has been carried out according to the standard   toxicological methods. Results. This paper   presents the data on the influence of pollutants of   various nature   (sodium hydrogen   phosphate,   sodium   sulfate, zinc complex of 2-Phosphono-1,2,4-butanetricarboxylic acid, dimethyl sulfide) on the Hyalella azteca Saussure, 1858 crustaceans.   Hyalella   azteca   have   found   a   wide   application   in   biotesting;   however, these crustaceans are not used in the system of fisheries regulation in Russia. The maximum allowable and threshold concentrations of the investigated substances in the aquatic environment   have   been established for Hyalella azteca using the experimental approach. The maximum allowable concentration (MAC) of sodium hydrogen phosphate in the phosphorus equivalent was 1.0 mg/L, its threshold concentration was 2.5 mg/L; the MAC of sodium sulfate in the sulfate anion equivalent was 750 mg/L, its threshold concentration was more than 750 mg/L; the MAC of the zinc complex of 2-Phosphono-1, 2,4-butanetricarboxylic acid was 10 mg/L, its threshold concentration was 100   mg/L;   the   MAC   of dimethyl sulfide was 10 mg/L, its threshold concentration was 20 mg/L. Conclusion. The amphipods

H.   azteca   are   moderately   sensitive   to   pollutants. Among   all   investigated   substances,   the   most   toxic for H. azteca was sodium hydrogen phosphate.

Keywords:   pollutants,    aquatic    environment,    maximum    allowable    concentration,    threshold concentration, crustaceans

New findings of medicinal leech Hirudo verbana (Carena, 1820) in the aquatic ecosystems of Kabardino-Balkaria    

Chernaya L.V., Kovalchuk L.A., Kozhaeva D.K., Zhantegolov D.V., Mikshevich N.V.


Background. Medicinal leeches are a traditional aquatic biological resource in Russia, officially used in practical medicine, pharmacology and cosmetology.   However,   in   recent   decades,   the   distribution range and abundance of these valuable aquatic organisms on the Russian territiry have decreased considerably. Relevance. The study of the present geographical range of this exploitable leech species is of practical importance, since the modern technology of their controlled breeding at biological production facilities requires constant replenishment of the broodstock and procurement of mature individuals from natural populations. This work is aimed at the identification of the current habitats of medicinal leeches in the aquatic ecosystems of the Kabardino-Balkarian Republic. Methods. This research was carried out in the last ten days of August 2021 in the daytime, in the littoral part of the water bodies of the plain and foothill zones of Kabardino-Balkaria. Leeches were caught with a handheld net, as well as collected manually from the objects in the immediate surroundings. The species of the caught leeches were determined using taxonomic keys in accordance with the modern classification. Results. New data on the distribution of the exploitable species of medicinal leeches Hirudo verbana (Carena, 1820) in the aquatic ecosystems of Kabardino-Balkaria are presented. Habitats of natural populations of this leech species were found in the Trek Lake and Deya River. Conclusion. The presence of the medicinal leech H. verbana in the investigated water bodies indicates their good ecological state. The results of this study make it possible to expand our understanding of the present distribution range of this exploitable species of aquatic organisms. Keywords:   distribution   range,   aquatic    ecosystems    of    Kabardino-Balkaria,    medicinal    leeches, exploitable species

Ichthyofauna of Marine and Inland Water Bodies

Distinctive features of the spawning migrations of the Caspian anadromous fish species through a sea shipping canal in 2001–2022    

Lepilina I.N., Konopleva I.V., Safaraliev I.A., Voynova T.V., Rakov A.M.


Background. Anadromous species of sturgeons, salmonids and clupeids inhabiting the Caspian Sea migrate to their spawning grounds predominantly through the Volga-Caspian Sea Shipping Canal (VCSSC), which hydrological and hydrographic features have an effect on the abundance dynamics of the anadromous fish species. Along the entire delta sea edge, the Volga-Caspian Sea Shipping Canal (around 200 km in length) provides the conditions required for fish migration. Relevance. The current decrease in the Caspian Sea level and extensive   accumulation   of   the fluvial and   marine   drift   sediments   impede navigation   due to insufficient depth of the waterway and affect the entering of the anadromous breeders. This work is aimed at the assessment of the VCSSC use by sheefish, black-spined herring, beluga sturgeon, Russian sturgeon and stellate sturgeon during various seasons within 2001–2022 time range. Methods. Trawling and net fishing have been conducted in the marine part of the canal. Based on the data from seine catches in the river, the seasonal dynamics   of fish migrations   has   been   identified. Results. Despite the existence of extensive riverine network   of the   River Volga   Delta suitable for the entering of anadromous fish species (Kirovsky, Belinsky, Igolkinsky, Karaysky and other banks),   the   Volga-Caspian   Sea   Shipping Canal (Main Bank) provides the anadromous fish species with a primary transit waterway   to   their spawning grounds—up to 90 % sheefish and black-backed herring   breeders out of   the   total   spawning stock abundance. The     share of stellate sturgeon migrating along this waterway is 76–78 %, for the Russian sturgeon it is 83–85 %, and for beluga sturgeon it is 90–95 % of the spawning populations in the year of their entering into the Volga River. In recent   years, the ecosystem of   the Volga River has been developing in the context of low water content, which leads to the decrease in the water level of fish   passage channels and tj a weakened flow, which,   exacerbated   by   abiotic factors, in   turn results in the reduction in the spawning stock abundance and decline in the yield of juveniles of the anadromous fish species [1]. Conclusion. During their pre-spawning season,   sturgeons,   sheefish   and   anadromous herring use the Volga-Caspian Sea Shipping Canal as the main migratory passageway to their spawning grounds. After the   spawning,   the   canal   provides   juveniles   and   mature   breeders   with   the   passageways to the feeding sites in the Northern Caspian Sea. Sturgeon juveniles and the individuals skipping the spawning actively feed in the marine part of the canal [2].

Keywords: Caspian Sea, anadromous species, spawning population, migrations, abundance, biomass

Distribution and abundance dynamics of the ichthyoplankton and early juveniles of Black and Caspian Sea sprat (tyulka) and European anchovy in the Azov Sea in 1993–2023    

Nadolinskiy R.V., Nadolinskiy V.P., Dudkin S.I.


Background. Natural and   anthropogenic   factors   have   a   major   influence   on   the   fish   population status. In the Azov Sea, for the last 30 years, substantial changes in the environment have been observed and new exotic species of aquatic living organisms have been introduced, which affected reproduction of fish species. Relevance. To identify the driving forces behind the present dynamics of population abundance of European anchovy and Black and Caspian Sea sprat (tyulka), it is crucial to summarize the data collected during the   annual   ichthyoplankton   surveys   of   the Azov   Sea.   This   work   is   aimed   at   the   assessment of the distribution of the ichthyoplankton and early juveniles of Black and Caspian Sea sprat (tyulka) and European anchovy in the Azov Sea area over time with regard to various environmental factors. Methods. Ichthyoplankton surveys are customarily conducted over the entirety of the Azov Sea in the middle of June, when eggs and larvae of Black and Caspian Sea sprat (tyulka) and European anchovy are caught at the highest quantities. Results. The dynamics of distribution and abundance variations for the early developmental stages of Black and Caspian Sea sprat (tyulka) and European anchovy under the influence of environmental factors, including ctenophores and scyphozoan jellyfishes, has been identified; a decrease in the survival rates of their jellyfishes juveniles in the present period of the Azov Sea salinization has been observed. Conclusion. In the context of   increasing salinity of the Azov Sea waters, the main factors contributing to the reduced survival rates of the Black and Caspian Sea sprat (tyulka) larvae and fingerlings are the decrease in spawning ground area and competition for food resources with scyphozoan jellyfishes and ctenophores. In the case of the European anchovy larvae and fingerlings, the main factor affecting their abundance is a small number of breeders; food competition and predation of the scyphozoan jellyfishes and ctenophores are of secondary importance.

Keywords: eggs, larvae, ichthyoplankton, Black and Caspian Sea sprat, European anchovy, distribution, abundance, salinization, jellyfish, Mnemiopsis leidyi, Azov Sea

Aquaculture and Methods of Artificial Reproduction

Dirstribution of antibiotic resistance genes in aquaculture targets and ecosystem components (review)    

Sedova D.A., Sazykina M.A., Zhuravlev P.V., Morozova M.A., Barabashin T.O., Sazykin I.S.


Introduction. Aquaculture is one of the sectors of food production in which antibiotics are widely used. Taking into account the global problem of increasing drug resistance   of   microorganisms,   aquaculture targets can serve as reservoirs for the accumulation, selection and distribution of antibiotic resistance genes (ARGs) and antibiotic resistant bacteria (ARB). Relevance. Currently, the ubiquity of antibiotic resistance genes is one of the threats to public health. Aim. This review discusses the results of studies of antibiotic resistance genes isolated both from various aquaculture environments (water, sediments, sewage) and from resistant bacteria that have etiological significance in the occurrence of diseases of commercial fish species, molluscs, crustaceans, etc. Methods. The analysis of both foreign and domestic publications has been carried out. In foreign studies, molecular genetic methods of detecting ARGs in total DNA samples of aquaculture targets are mainly used. The metagenomic quantification of the bacterial communities and ARGs by sequencing is the most indicative one. Results. Despite the relevance of the topic, there are no available publications that are directly devoted to the resistomes in the targets of the Russian aquaculture. Basically, the study of antibiotic resistance in this area focuses only on the phenotypic manifestations of   the   resistance   of   fish   pathogenic   bacteria   to   the   antibiotics   widely   used in the Russian aquaculture. Conclusion. Accumulated research results on this subject matter are quite extensive; their summarization within a single review article might facilitate the further work in this area. Keywords: antibiotic resistance genes, antibiotic resistant   bacteria,   aquaculture,   antibiotics,   horizontal gene transfer

Fisheries and Processing of Aquatic Bioresources

Development of the mixed fish- and plant-based concentrated food products (snacks) derived from the fish caught in inland water bodies    

Syromyatnikov I.A., Ivanova E.E., Chibich N.V.


Introduction. Concentrated food products are in great demand for their lengthy storage time,   high nutritional value and full readiness for consumption. The range of existing concentrated food products derived from fish is limited by the presence of intermuscular bones, high rates of fish oil oxidization, and by pronounced specific taste and flavor, usually undesirable by the consumers. These reasons contribute to the relevance   of   the   exploration   of   possible   ways   to   improve   marketability   and   consumer   appeal of minced fish and the development of recipes for the mixed fish- and plant-based concentrated food products derived from it. This work is aimed at the development of formulations for the mixed fish- and plant-based concentrated food products (snacks) derived from preprocessed fish mince with improved organoleptic and rheological properties. Over the course of this   research,   established   and   currently accepted research methods have been used. Results. It has been found out that rinsing fish mince with

1 % aqueous solution (aqueous system) based on mustard powder allows for the improvement   of its texture, color, flavor and taste. With the use of mathematical modeling, optimal proportions (by weight) for the main components of the mixed fish- and plant-based concentrated food products have been identified: fish mince (70–85 %), wheat bran (9–10 %), carrot powder (5 %), and beet powder (10 %). Conclusion. It has been established that 1 % aqueous solution (aqueous system) of mustard powder can be used as a rinsing solution for fish mince to ensure its fresh flavor with pleasant mustard undertones, as well as vastly improved color and texture. The optimized weight percentages of the main components (fish mince, wheat bran, powdered carrot and beet) made it possible to obtain concentrated food products with attractive organoleptic properties.

Keywords: mixed fish- and plant-based concentrated food products, snacks, recipe, rinsing, fish mince, organoleptic properties

Informational Messages

On the upcoming 4th International Scientific and Practical Conference “Biological Diversity: Study, Conservation, Restoration, Rational Use”, dedicated to the 110th anniversary of the foundation of T.I. Vyazemsky Karadag Scientific Station, 17–22 September, 2024 (Kerch State Maritime Technological University, Kerch, Russia)